si rna against p53 (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
si rna against p53
Si Rna Against P53, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 9281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/si+rna/p53/pm41594681-62-11-17
Average 96 stars, based on 9281 article reviews
Si Rna Against P53, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 9281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/si+rna/p53/pm41594681-62-11-17
Average 96 stars, based on 9281 article reviews
si rna against p53 - by Bioz Stars,
2026-10
96/100 stars
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Targeted Gene Expression:Article Title: Stress hormone signalling inhibits Th1 polarization in a CD4 T‐cell‐intrinsic manner via mTORC1 and the circadian gene PER1 Article Snippet: .. Targeted gene expression ( PER1 , PER2 , PER3 ) was knocked down as described elsewhere [ ], in up to 5 × 10 6 cells using the P3 Primary Cell 4D‐Nucleofector X Kit L (V4XP‐3024, Lonza) with 90 μl P3 primary cell solution and 100 pmol of corresponding Control:Article Title: Stress hormone signalling inhibits Th1 polarization in a CD4 T‐cell‐intrinsic manner via mTORC1 and the circadian gene PER1 Article Snippet: .. Targeted gene expression ( PER1 , PER2 , PER3 ) was knocked down as described elsewhere [ ], in up to 5 × 10 6 cells using the P3 Primary Cell 4D‐Nucleofector X Kit L (V4XP‐3024, Lonza) with 90 μl P3 primary cell solution and 100 pmol of corresponding Article Title: Long non-coding RNA cancer susceptibility candidate 2 regulates the function of human fibroblast-like synoviocytes via the microRNA-18a-5p/B-cell translocation gene 3 signaling axis in rheumatoid arthritis Article Snippet: .. The control plasmid, lncRNA CASC2 plasmid, mimic control (cat. no. miR1N0000001-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), miR-18a-5p mimic (cat. no. miR10000072-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), inhibitor control (cat. no. miR2N0000001-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), miR-18a-5p inhibitor (cat. no. miR20000072-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), control small interfering ( Article Title: MicroRNA-221 regulates cell activity and apoptosis in acute lymphoblastic leukemia via regulating PTEN Article Snippet: Finally, ECL reagent (EMD Millipore) was used to visualize protein bands using FluorChem FC3 (ProteinSimple), and AlphaView 3.4.0 software (ProteinSimple) was used for semi-quantification. .. Jurkat cells were seeded into 6-well plates (1x10 6 cells/well) and cultured at 37 ̊C for 24 h. Then, cells were transfected with 100 nM inhibitor control (the negative control of miR-221 inhibitor; 5'-CAGUACUUUUGUGUAGUACAA-3'; Guangzhou Ribobio Co., Ltd.), 100 nM miR-221 inhibitor (miR-221 antagonist' 5'-GAAACCCAGCAGACAAUGUAGCU-3'; Guangzhou Ribobio Co., Ltd.), 50 nM mimic control (the negative control of miR-221 mimic; 5'-CGGUACGAUCGCGGCGGGAUAUC-3'; Guangzhou Ribobio Co., Ltd.), 50 nM miR-221 mimic (miR-221 agonist; 5'-AGCUACAUUGUCUGCUGGGUUUC-3'; Guangzhou Ribobio Co., Ltd.), 100 nM miR-221 inhibitor + 0.2 μM control-small interfering ( Article Title: Identification of VIMP as a gene inhibiting cytokine production in human CD4+ effector T cells Article Snippet: The EBV-B cells were irradiated in RS2000 X-Ray Biological Irradiator (Rad Source Technologies) for 30 min with a total of 90 Gy. .. Targeted gene’s expression was knocked-down in up to 5 x 10 6 cells using the P3 Primary Cell 4D-Nucleofector X Kit L (V4XP-3024, Lonza) with 90 μl P3 Primary cell solution and 100 pmol of corresponding Transfection:Article Title: Stress hormone signalling inhibits Th1 polarization in a CD4 T‐cell‐intrinsic manner via mTORC1 and the circadian gene PER1 Article Snippet: .. Targeted gene expression ( PER1 , PER2 , PER3 ) was knocked down as described elsewhere [ ], in up to 5 × 10 6 cells using the P3 Primary Cell 4D‐Nucleofector X Kit L (V4XP‐3024, Lonza) with 90 μl P3 primary cell solution and 100 pmol of corresponding Article Title: Long non-coding RNA cancer susceptibility candidate 2 regulates the function of human fibroblast-like synoviocytes via the microRNA-18a-5p/B-cell translocation gene 3 signaling axis in rheumatoid arthritis Article Snippet: .. The control plasmid, lncRNA CASC2 plasmid, mimic control (cat. no. miR1N0000001-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), miR-18a-5p mimic (cat. no. miR10000072-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), inhibitor control (cat. no. miR2N0000001-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), miR-18a-5p inhibitor (cat. no. miR20000072-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), control small interfering ( Article Title: MicroRNA-221 regulates cell activity and apoptosis in acute lymphoblastic leukemia via regulating PTEN Article Snippet: Finally, ECL reagent (EMD Millipore) was used to visualize protein bands using FluorChem FC3 (ProteinSimple), and AlphaView 3.4.0 software (ProteinSimple) was used for semi-quantification. .. Jurkat cells were seeded into 6-well plates (1x10 6 cells/well) and cultured at 37 ̊C for 24 h. Then, cells were transfected with 100 nM inhibitor control (the negative control of miR-221 inhibitor; 5'-CAGUACUUUUGUGUAGUACAA-3'; Guangzhou Ribobio Co., Ltd.), 100 nM miR-221 inhibitor (miR-221 antagonist' 5'-GAAACCCAGCAGACAAUGUAGCU-3'; Guangzhou Ribobio Co., Ltd.), 50 nM mimic control (the negative control of miR-221 mimic; 5'-CGGUACGAUCGCGGCGGGAUAUC-3'; Guangzhou Ribobio Co., Ltd.), 50 nM miR-221 mimic (miR-221 agonist; 5'-AGCUACAUUGUCUGCUGGGUUUC-3'; Guangzhou Ribobio Co., Ltd.), 100 nM miR-221 inhibitor + 0.2 μM control-small interfering ( Plasmid Preparation:Article Title: Long non-coding RNA cancer susceptibility candidate 2 regulates the function of human fibroblast-like synoviocytes via the microRNA-18a-5p/B-cell translocation gene 3 signaling axis in rheumatoid arthritis Article Snippet: .. The control plasmid, lncRNA CASC2 plasmid, mimic control (cat. no. miR1N0000001-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), miR-18a-5p mimic (cat. no. miR10000072-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), inhibitor control (cat. no. miR2N0000001-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), miR-18a-5p inhibitor (cat. no. miR20000072-1-5; Guangzhou RiboBio Co., Ltd., Guangzhou, China), control small interfering ( Cell Culture:Article Title: MicroRNA-221 regulates cell activity and apoptosis in acute lymphoblastic leukemia via regulating PTEN Article Snippet: Finally, ECL reagent (EMD Millipore) was used to visualize protein bands using FluorChem FC3 (ProteinSimple), and AlphaView 3.4.0 software (ProteinSimple) was used for semi-quantification. .. Jurkat cells were seeded into 6-well plates (1x10 6 cells/well) and cultured at 37 ̊C for 24 h. Then, cells were transfected with 100 nM inhibitor control (the negative control of miR-221 inhibitor; 5'-CAGUACUUUUGUGUAGUACAA-3'; Guangzhou Ribobio Co., Ltd.), 100 nM miR-221 inhibitor (miR-221 antagonist' 5'-GAAACCCAGCAGACAAUGUAGCU-3'; Guangzhou Ribobio Co., Ltd.), 50 nM mimic control (the negative control of miR-221 mimic; 5'-CGGUACGAUCGCGGCGGGAUAUC-3'; Guangzhou Ribobio Co., Ltd.), 50 nM miR-221 mimic (miR-221 agonist; 5'-AGCUACAUUGUCUGCUGGGUUUC-3'; Guangzhou Ribobio Co., Ltd.), 100 nM miR-221 inhibitor + 0.2 μM control-small interfering ( Negative Control:Article Title: MicroRNA-221 regulates cell activity and apoptosis in acute lymphoblastic leukemia via regulating PTEN Article Snippet: Finally, ECL reagent (EMD Millipore) was used to visualize protein bands using FluorChem FC3 (ProteinSimple), and AlphaView 3.4.0 software (ProteinSimple) was used for semi-quantification. .. Jurkat cells were seeded into 6-well plates (1x10 6 cells/well) and cultured at 37 ̊C for 24 h. Then, cells were transfected with 100 nM inhibitor control (the negative control of miR-221 inhibitor; 5'-CAGUACUUUUGUGUAGUACAA-3'; Guangzhou Ribobio Co., Ltd.), 100 nM miR-221 inhibitor (miR-221 antagonist' 5'-GAAACCCAGCAGACAAUGUAGCU-3'; Guangzhou Ribobio Co., Ltd.), 50 nM mimic control (the negative control of miR-221 mimic; 5'-CGGUACGAUCGCGGCGGGAUAUC-3'; Guangzhou Ribobio Co., Ltd.), 50 nM miR-221 mimic (miR-221 agonist; 5'-AGCUACAUUGUCUGCUGGGUUUC-3'; Guangzhou Ribobio Co., Ltd.), 100 nM miR-221 inhibitor + 0.2 μM control-small interfering ( Article Title: Interferon-induced protein with tetratricopeptide repeats 3 may be a key factor in primary biliary cholangitis Article Snippet: Microarray data are deposited in the National Center for Biotechnology Information Gene Expression Omnibus database (accession number GSE168052). .. Validated Viability Assay:Article Title: Diospyros lotus leaf extract and its main component myricitrin inhibit itch‑related IL‑6 and IL‑31 by suppressing microglial inflammation and microglial‑mediated astrocyte activation Article Snippet: .. The following reagents and materials were purchased from the specified suppliers: LPS, Griess reagent and protease inhibitors from MilliporeSigma; myricitrin from Tokyo Chemical Industry; Quanti-MAX WST-8 Cell Viability Assay Kit and WestGlow FEMTO Chemiluminescent substrate from Biomax Ltd.; radio-immunoprecipitation assay (RIPA) buffer, IL-33, inositol 1,4,5-trisphosphate receptor 1 (IP3R1), lipocalin-2 (LCN2), glial fibrillary acidic protein (GFAP), goat anti-rabbit IgG Alexa Fluor 488 antibodies and goat anti-mouse IgG Alexa Fluor 488 from Thermo Fisher Scientific, Inc.; IL-31 antibodies from Abcam; β-actin, phosphorylated (p-)IκBα, IκBα, p-NF-κB, NF-κB, p-JNK, JNK, p-p38, p38, oncostatin M receptor (OSMR), Toll-like receptor 4 (TLR4), IL-6, interleukin 31 receptor a (IL31RA) antibodies and IL-31 short interfering ( Radio Immunoprecipitation:Article Title: Diospyros lotus leaf extract and its main component myricitrin inhibit itch‑related IL‑6 and IL‑31 by suppressing microglial inflammation and microglial‑mediated astrocyte activation Article Snippet: .. The following reagents and materials were purchased from the specified suppliers: LPS, Griess reagent and protease inhibitors from MilliporeSigma; myricitrin from Tokyo Chemical Industry; Quanti-MAX WST-8 Cell Viability Assay Kit and WestGlow FEMTO Chemiluminescent substrate from Biomax Ltd.; radio-immunoprecipitation assay (RIPA) buffer, IL-33, inositol 1,4,5-trisphosphate receptor 1 (IP3R1), lipocalin-2 (LCN2), glial fibrillary acidic protein (GFAP), goat anti-rabbit IgG Alexa Fluor 488 antibodies and goat anti-mouse IgG Alexa Fluor 488 from Thermo Fisher Scientific, Inc.; IL-31 antibodies from Abcam; β-actin, phosphorylated (p-)IκBα, IκBα, p-NF-κB, NF-κB, p-JNK, JNK, p-p38, p38, oncostatin M receptor (OSMR), Toll-like receptor 4 (TLR4), IL-6, interleukin 31 receptor a (IL31RA) antibodies and IL-31 short interfering ( Expressing:Article Title: Identification of VIMP as a gene inhibiting cytokine production in human CD4+ effector T cells Article Snippet: The EBV-B cells were irradiated in RS2000 X-Ray Biological Irradiator (Rad Source Technologies) for 30 min with a total of 90 Gy. .. Targeted gene’s expression was knocked-down in up to 5 x 10 6 cells using the P3 Primary Cell 4D-Nucleofector X Kit L (V4XP-3024, Lonza) with 90 μl P3 Primary cell solution and 100 pmol of corresponding |
